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rabbit anti calpain 1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit anti calpain 1
    Rabbit Anti Calpain 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 186 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+calpain+1/Calpain+1+Large+Subunit+(Mu-type)+Antibody/bio_rxiv__64898__2026__02__23__707462-123-50-55
    Average 95 stars, based on 186 article reviews
    rabbit anti calpain 1 - by Bioz Stars, 2026-10
    95/100 stars

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    Article Title: Skeletal muscle myosin heavy chain fragmentation following exercise may be linked to post‐exercise inflammation and remodelling
    Article Snippet: Primary antibodies included:mouse anti-MyHC (Developmental StudiesHybridomaBank; Iowa City, IA, USA; cat. no.: A4.1025), rabbit anti-αβ-crystallin (CRYAB; Cell Signaling Technology, Danvers, MA, USA; cat. no.: 45844), rabbit anti-HSP90 (Cell Signaling Technology, cat. no.: 4874), rabbit antiHSP70 (Cell Signaling Technology, cat. no.: 4872), rabbit anti-20S proteasome core subunits (Enzo, Long Island, NY, USA; cat. no.: BMLPW8155), rabbit anti-calpain-1 (Calp-1; Cell Signaling Technology, cat. no.: 2556), rabbit anti-calpain-2 (Calp-2; Cell Signaling Technology, cat. no.: 2556), rabbit anti-ubiquitin (Cell Signaling Technology, cat. no.: 58395), rabbit anti-p-p70S6K (Thr389; Cell Signaling Technology, cat. no.: 34475), and rabbit anti-p-MKK3b (Ser189; Cell Signaling Technology, cat. no.: 9236).

    Article Title: Vascular FLRT2 regulates venous-mediated angiogenic expansion and CNS barriergenesis
    Article Snippet: The following primary antibodies were used: mouse anti-VE-cadherin 1:100 (sc-9989, Santa Cruz), mouse anti-VE-cadherin 1:100 (MABT134, Millipore), goat anti-VE-cadherin 1:100 (AF1002, R&D), rabbit anti-Calpain-2 1:100 (ab39165, Abcam), rabbit anti-Calpain-1 1:100 (2556, Cell signaling), goat anti-FLRT2 1:50 (AF2877, R&D), rabbit anti-Numb 1:100 (2756, Cell Signaling), mouse anti-GM130 1:100 (610823, BD Biosciences) and rat anti-Pecam1 1:100 (550274, BD Pharmingen).

    Incubation:

    Article Title: Glucose shields RINm5F beta cells against arsenic-induced apoptosis
    Article Snippet: Proteins were transferred to PVDF membranes using a semi-dry transfer system (Bio-Rad, USA) at 20 V for 60 min. Membranes were blocked with TBS-tween containing 4% Blotto non-fat dry milk (Santa Cruz Biotechnology, sc-2325, USA) for 1 h at room temperature. .. Primary antibodies were diluted in TBS-Tween 20 with 4% milk and incubated overnight at 4 °C with rabbit anti-p-Akt (Ser473) (#9271, DF 1:500, Cell Signaling), rabbit anti-Akt (#9272, DF 1:2000, Cell Signaling), rabbit anti-p-P70S6K (Thr389) (#9205, DF 1:500, Cell Signaling), rabbit anti-P70S6K (#9202, DF 1:1000, Cell Signaling), rabbit anti-p-IRS1 (S1101) (#2385, DF 1:1000, Cell Signaling), rabbit anti-IRS1 (#2382, DF 1:1000, Cell Signaling), mouse anti-p-ERK 1/2 (T202/Y204.22 A) (SC-136521, DF 1:200, Santa Cruz Biotechnology), mouse anti-ERK 1/2 (SC-514302, DF 1:1000, Santa Cruz Biotechnology), rabbit anti-cleaved caspase-3 (N175) (#9661, DF 1:1000, Cell Signaling), mouse anti-BcL-xL (SC-8392, DF 1:1000, Santa Cruz Biotechnology), rabbit anti-Bax (SC-493, DF 1:1000, Santa Cruz Biotechnology), rabbit anti-calpain-1 (#2556, DF 1:1000, Cell Signaling), rabbit anti-calpain-2 (ab126600, DF 1:1000, Abcam), rabbit anti-calpain-10 (ab28226, DF 1:1000, Abcam), rabbit anti-αII spectrin (BML-FG 6090 − 0100, DF 1:1000, ENZO).Secondary antibody: Peroxidase-conjugated mouse anti-rabbit IgG (light chain specific, Jackson ImmunoResearch #211-032-171, DF 1:6000) and peroxidase-conjugated affinipure goat anti-mouse IgG (Jackson ImmunoResearch #115-035-146; DF 1:4000). .. Protein bands were detected using the ECL Prime kit (Amersham, UK) and visualized with a Li-Cor C-Digit scanner.

    Article Title: Large increases in resistance training volume do not impair skeletal muscle hypertrophy or anabolic–catabolic molecular signalling in trained individuals
    Article Snippet: .. The membranes were incubated overnight at 4°C with the following antibodies at a dilution of 1:1000 in TBST with 5% bovine serum albumin (BSA): rabbit anti-MyHC (cat. no: 64038, Cell Signaling Technology); rabbit anti-polyubiquitin (cat. no: 3933, Cell Signaling Technology); rabbit 20S antibody cocktail (cat. no: PW8155, Enzo Life Sciences); rabbit anti-calpain-1 (cat. no: 2556, Cell Signaling Technology); rabbit anti-calpain-2 (cat. no: 70655, Cell Signaling Technology); rabbit anti-LC3 (cat. no: 2775, Cell Signaling Technology); rabbit anti-FOXO1 (cat. no: 9454, Cell Signaling Technology); rabbit anti-FOXO3 (cat. no: 24975, Cell Signaling Technology); rabbit anti-RPS6 (cat. no: 2217, Cell Signaling Technology); rabbit anti-4EBP1 (cat. no: 9644, Cell Signaling Technology); rabbit anti-phospho-4EBP1 (cat. no: 2855, Cell Signaling Technology); rabbit anti-p62 (cat. no: 5114, Cell Signaling Technology); mouse anti-SKIV2L2 (cat. no: sc-515828, Santa Cruz Technology); mouse anti-G3BP1 (cat. no: sc-365338, Santa Cruz Technology); rabbit anti-p70S6K (cat. no: 9234, Cell Signaling Technology); rabbit anti-phospho-p70S6K (cat. no: 2983, Cell Signaling Technology); rabbit anti-mTOR (cat. no: 5536, Cell Signaling Technology); rabbit anti-phospho-mTOR (cat. no: 2971, Cell Signaling Technology). ..

    Article Title: Sucrose but not arsenic induce hepatic steatosis which correlates with calpain-1 inhibition
    Article Snippet: Proteins were transferred to PVDF membranes in a semi-dry chamber (BioRad) at 20 V for 50 min. Membranes were blocked with tris buffered saline with 0.1% Tween 20 (TBS-T) and 4% low-fat milk for Western blot (Santa Cruz Biotechnology). .. The primary antibodies were incubated overnight at 4 °C: rabbit anti-calpain-1 (#2556, 1:1000, Cell Signaling), rabbit anti-calpain-2 (ab126600, 1:1000, Abcam), rabbit anti-calpain small subunit 1 (A307324, 1:1000, antibodies.com), rabbit anti-calpastatin (ab28252, 1:1000, Abcam), rabbit anti-calpain-10 (ab28226, 1:1000, Abcam), rabbit anti-αII spectrin (PA5–35383, 1:1000, Invitrogen), rabbit anti-PPARγ (PA3-821A, 1:1000, Invitrogen). .. The secondary antibody used was the Peroxidase IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, light chain specific (211-032-171, 1:6000, Jackson Immunoresearch).

    Article Title: Individual muscle hypertrophy response is affected by the overload progression model and is associated with changes in satellite cell content.
    Article Snippet: Purpose We aimed to compare individual hypertrophic responses to resistance training in which overload progressed either by adjusting the load (LOADProg) or by increasing the number of repetitions (REPSProg).. Furthermore, we investigated whether greater responsiveness to one protocol was associated with chronic changes in myonuclei and satellite cells, proteolysis and extracellular matrix (ECM) remodeling biomarkers.. Methods Thirty-seven untrained participants had their legs randomized into LOADProg and REPSProg and underwent 10 weeks of training.

    Article Title: Sucrose but not arsenic induce hepatic steatosis which correlates with calpain-1 inhibition.
    Article Snippet: Proteins were transferred to PVDF membranes in a semi-dry chamber (BioRad) at 20 V for 50 min. Membranes were blocked with tris buffered saline with 0.1% Tween 20 (TBS-T) and 4% low-fat milk for Western blot (Santa Cruz Biotechnology). .. The primary antibodies were incubated overnight at 4 °C: rabbit anti-calpain-1 (#2556, 1:1000, Cell Signaling), rabbit anti-calpain-2 (ab126600, 1:1000, Abcam), rabbit anti-calpain small subunit 1 (A307324, 1:1000, antibodies.com), rabbit anti-calpastatin (ab28252, 1:1000, Abcam), rabbit anti-calpain-10 (ab28226, 1:1000, Abcam), rabbit anti-αII spectrin (PA5–35383, 1:1000, Invitrogen), rabbit anti-PPARγ (PA3-821A, 1:1000, Invitrogen). .. The secondary antibody used was the Peroxidase IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, light chain specific (211-032-171, 1:6000, Jackson Immunoresearch).

    Article Title: Glucose shields RINm5F beta cells against arsenic-induced apoptosis.
    Article Snippet: Proteins were transferred to PVDF membranes using a semi-dry transfer system (Bio-Rad, USA) at 20 V for 60 min. Membranes were blocked with TBS-tween containing 4% Blotto non-fat dry milk (Santa Cruz Biotechnology, sc-2325, USA) for 1 h at room temperature. .. Primary antibodies were diluted in TBSTween 20 with 4% milk and incubated overnight at 4 °C with rabbit anti-p-Akt (Ser473) (#9271, DF 1:500, Cell Signaling), rabbit anti-Akt (#9272, DF 1:2000, Cell Signaling), rabbit anti-p-P70S6K (Thr389) (#9205, DF 1:500, Cell Signaling), rabbit anti-P70S6K (#9202, DF 1:1000, Cell Signaling), rabbit anti-p-IRS1 (S1101) (#2385, DF 1:1000, Cell Signaling), rabbit anti-IRS1 (#2382, DF 1:1000, Cell Signaling), mouse anti-p-ERK 1/2 (T202/ Y204.22 A) (SC-136521, DF 1:200, Santa Cruz Biotechnology), mouse anti-ERK 1/2 (SC-514302, DF 1:1000, Santa Cruz Biotechnology), rabbit anti-cleaved caspase-3 (N175) (#9661, DF 1:1000, Cell Signaling), mouse anti-BcL-xL (SC-8392, DF 1:1000, Santa Cruz Biotechnology), rabbit anti-Bax (SC-493, DF 1:1000, Santa Cruz Biotechnology), rabbit anti-calpain-1 (#2556, DF 1:1000, Cell Signaling), rabbit anti-calpain-2 (ab126600, DF 1:1000, Abcam), rabbit anti-calpain-10 (ab28226, DF 1:1000, Abcam), rabbit anti-αII spectrin (BML-FG 6090 − 0100, DF 1:1000, ENZO).Secondary antibody: Peroxidase-conjugated mouse anti-rabbit IgG (light chain specific, Jackson ImmunoResearch #211-032-171, DF 1:6000) and peroxidase-conjugated affinipure goat antimouse IgG (Jackson ImmunoResearch #115-035-146; DF 1:4000). .. Protein bands were detected using the ECL Prime kit (Amersham, UK) and visualized with a Li-Cor C-Digit scanner.



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